Halosperm is an in vitro diagnostic kit that allows the measurement of DNA fragmentation in a fast, easy, and reproducible manner, without the need for complex laboratory equipment. Halosperm allows for an improved evaluation of sperm quality, complementing the information offered by the conventional sperm analysis. Conventional semen analysis only studies spermatozoa concentration, motility and morphology. It is an incomplete study, as it omits analysis of one of the most important parameters, the integrity of the DNA molecule. 15% of men considered to be infertile present normal sperm analyses, which makes a deeper assessment of the sample important. Halosperm facilitates decision-making for medical professionals regarding which assisted reproduction technique is most appropriate for each couple.
Halosperm is based on the SCD technique, patented by Halotech, based on a controlled DNA denaturation process to facilitate the subsequent removal of the proteins contained in each spermatozoon. In this way, normal spermatozoa create halos formed by loops of DNA at the head of the sperm, which are not present in those with damaged DNA.
The images of halos generated with Halosperm® are highly contrasted and can be evaluated precisely using conventional or fluorescent microscopy. The tails of the spermatozoa are preserved, so it is possible to discriminate them from other cell types that might be present in the ejaculate, such as leukocytes. In addition, Halosperm® allows the visualisation of spermatozoa that contain highly degraded DNA compared to other types of less aggressive damage. This fact is of great importance in the diagnosis and monitoring of varicocele.
Medical professionals use information from Halotech solutions at the start of treatment, supplementing conventional semen analysis. Sperm DNA damage is a multifactorial process. Because it can have multiple causes, fragmentation, oxidation, and vitality analysis is especially recommended for:
Natural factors like improper maturation or oxidative stress translate into failures during the production of spermatozoa in the testicle.
Halosperm G2® measures DNA fragmentation in a fast, easy way, similar to Halosperm, but also partially eliminates the bad odour generated by the lysis. By using a few drops, the test will offer the same result as the Halosperm test, maintaining its simplicity and ease of use.
Halosperm G2® also includes staining solutions for bright field microscopy.